BD TSB broth만들기

ATCC Medium: 18 Tryptic Soy Agar/Broth (Soybean-Casein Digest Medium, USP) Agar Medium Tryptic Soy Agar (BD 236950)…………………..40 g DI Water……………………………………………1000 ml Autoclave at 121º C. Broth Medium Tryptic Soy Broth (BD cat 211825)…………….30 g DI Water…………………………………………..1000 ml Autoclave at 121º C. *This product is commercially available from BD. To make medium from scratch, follow formulation below: Tryptic Soy Agar Composition Tryptone………………………………….………17 g Soytone…………………………………………..3 g Dextrose………………………………….………2.5 g NaCl………………………………………..……..5.0 g K2HPO4……………………………………..…...2.5 g Agar………………………………………..……..15 g Final pH 7.3 +/- 0.2. *Omit agar for broth medium. It should be noted that this formulation contains dextrose. The commercially available Tryptic Soy Agars do not contain dextrose

 

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